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Related Concept Videos

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Microbial Growth Measurement: Direct Methods

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Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
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Related Experiment Video

Updated: Jun 10, 2025

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Oral Bacteria Counter Using Dielectrophoretic Impedance Measurement: Usefulness and Usage Considerations.

Akira Imakiire1, Sakiko Soutome1, Yuichi Nakamura1

  • 1Department of Oral Health, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JPN.

Cureus
|October 17, 2024
PubMed
Summary

The Oral Bacteria Counter may overestimate bacterial counts due to disinfectants and electrolytes, and underestimate them with increased viscosity. Accurate oral bacteria quantification requires considering these factors and using complementary methods like DR-PCR.

Keywords:
bacterial culturebenzethonium chloridechlorhexidinedelayed real-time pcrdielectrophoretic impedance measurementmicroorganism quantitative analyzeroral bacteriaoral bacteria counteroral carestaphylococcus aureus

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Area of Science:

  • Microbiology
  • Biomedical Engineering
  • Dental Diagnostics

Background:

  • The oral cavity harbors bacteria linked to systemic diseases.
  • The Oral Bacteria Counter (PHC Corporation) offers rapid bacterial counting using dielectrophoretic impedance measurements.
  • A limitation is its potential to detect nonviable bacteria.

Purpose of the Study:

  • To assess the influence of disinfectants, electrolytes, and viscosity on the Oral Bacteria Counter's accuracy.
  • To determine if the device quantifies nonviable bacteria similarly to viable ones.

Main Methods:

  • Evaluated disinfectants (povidone-iodine, benzethonium chloride, chlorhexidine) and saline using the Oral Bacteria Counter.
  • Assessed viscosity effects by mixing sterile water with glycerol.
  • Compared Oral Bacteria Counter results with delayed real-time polymerase chain reaction (DR-PCR) and culture methods for viable bacteria quantification.

Main Results:

  • High readings were observed with disinfectants (5% CHX, 7% PV-I) even without viable bacteria.
  • Increased viscosity from glycerol led to decreased bacterial counts.
  • Electrolytes, especially saline, caused higher readings, with the device detecting both viable and nonviable bacteria.

Conclusions:

  • Disinfectants, viscosity, and electrolytes can impact the Oral Bacteria Counter's accuracy.
  • Combining Oral Bacteria Counter data with methods like DR-PCR is recommended for reliable oral bacteria assessment.