Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Types Of Column Chromatography01:29

Types Of Column Chromatography

11.0K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
11.0K
Affinity Chromatography01:03

Affinity Chromatography

551
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
551

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Life-death trade-offs: HSV-1 ICP27 differentially modulates intrinsic apoptotic signaling in epithelial and neuron-like cells.

Frontiers in microbiology·2026
Same author

Impact of chemical structure and substituents of polysaccharide-based chiral stationary phases on cannabinoids retention under normal phase conditions.

Analytical and bioanalytical chemistry·2026
Same author

Investigation of adsorption behavior and scale up for hydroxysafflor yellow A purification from Safflower: a case study.

Journal of chromatography. A·2026
Same author

Volatilomic Differentiation of Protected-Origin Italian Balsamic Vinegars by HS-SPME-GC×GC-TOFMS.

Journal of separation science·2026
Same author

Diving into AA9-mediated transglutaminase 2 inhibition reveals ferroptosis as driver of anticancer effects.

Biochemical pharmacology·2026
Same author

Exploratory Volatilome Profiling of Inflammation in Skin Fibroblasts: A Proof-of-Concept Study.

International journal of molecular sciences·2026

Related Experiment Video

Updated: Jun 10, 2025

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

11.1K

Integrated multidimensional chromatography on preparative scale for oligonucleotides purification.

Chiara Nosengo1, Desiree Bozza1, Giulio Lievore2

  • 1Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, Ferrara 44121, Italy.

Journal of Chromatography. A
|October 17, 2024
PubMed
Summary
This summary is machine-generated.

An integrated batch process for oligonucleotide purification combines two chromatography steps, reducing production time and improving reproducibility. This automated method enhances purity and yield for therapeutic nucleic acid manufacturing.

Keywords:
Active pharmaceutical ingredientsAnion exchange chromatographyMultidimensional chromatographyOligonucleotidesPreparative chromatographyReversed phase chromatography

More Related Videos

Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

3.3K
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

4.2K

Related Experiment Videos

Last Updated: Jun 10, 2025

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

11.1K
Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

3.3K
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

4.2K

Area of Science:

  • Pharmaceutical Sciences
  • Biotechnology
  • Analytical Chemistry

Background:

  • Therapeutic oligonucleotides offer specific gene expression regulation for disease treatment.
  • High purity Active Pharmaceutical Ingredients (APIs) are crucial for oligonucleotide therapeutics, necessitating intensive purification.
  • Preparative liquid chromatography, particularly Anion Exchange (AEX) and Ion Pair Reversed Phase (IP-RP) chromatography, is standard for large-scale oligonucleotide purification.

Purpose of the Study:

  • To evaluate an "Integrated Batch process" for purifying single-stranded DNA oligonucleotides on a (semi)preparative scale.
  • To compare the performance of the integrated approach against traditional single batch chromatography runs.
  • To demonstrate the potential of multidimensional and orthogonal chromatography for enhanced oligonucleotide purification.

Main Methods:

  • Implementation of an "Integrated Batch process" combining two sequential chromatographic steps (AEX and IP-RP) without intermediate hold or sampling.
  • Purification of a single-stranded DNA oligonucleotide using the integrated approach.
  • Comparison of performance parameters (purity, yield, time) against conventional single batch runs under varied experimental conditions.

Main Results:

  • The Integrated Batch process demonstrated significant potential for oligonucleotide purification.
  • This approach offers a reduction in overall production time compared to sequential single batch purifications.
  • The integrated method enhances the robustness and reproducibility of the purification process due to high automation.

Conclusions:

  • The "Integrated Batch process" presents a viable and efficient strategy for the downstream processing of therapeutic oligonucleotides.
  • This automated, multidimensional chromatographic approach addresses the purity-yield trade-off inherent in single-step purifications.
  • The study highlights the feasibility of integrating orthogonal chromatographic techniques to streamline and improve the manufacturing of oligonucleotide-based therapeutics.