Related Experiment Video
Updated: Jun 10, 2025
An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur
Published on: October 23, 2017
Autologous ADSCs with exogenous NPY promotes fracture healing in ovariectomized rats
Hong Liu1, Jiaoyang Wu2, Huixuan Wu3
1Department of Nutrition, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China.
Objective:
To evaluate the role of autologous adipose-derived stem cells (ADSCs) with exogenous neuropeptide Y (NPY) on osteoporotic fracture healing.
Methods:
We established a fracture healing model in ovariectomized rats of osteoporosis. Autologous ADSCs, isolated from rats' subcutaneous adipose tissue, with exogenous NPY were transplanted into the fracture site four times a week. The fracture healing was observiked with X-ray diagnostic techniques, and the rats' tibias were taken out for section-staining and micro-CT scanning 3 and 6 weeks after fracture. Next, we explored the underlying mechanism by which the transplantation contributed to fracture healing.
Results:
Co-culture and co-transplantation of ADSCs with NPY could stimulate the osteoporotic fracture healing. NPY may promote the osteogenic differentiation of ADSCs through Y1 receptor.
Conclusion:
This result provides a platform for the development of novel therapies for bone regeneration with endogenous ADSCs.
