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Related Experiment Video

Updated: Jun 10, 2025

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FLUID: a fluorescence-friendly lipid-compatible ultrafast clearing method.

Zizheng Wang1, Xiao Xiao1,2, Ziwen Zhou1

  • 1Department of Psychiatry of the First Affiliated Hospital, Zhejiang University School of Medicine, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310058, China.

Biomedical Optics Express
|October 18, 2024
PubMed
Summary
This summary is machine-generated.

We developed FLUID, a rapid, water-based tissue clearing method that preserves lipids and fluorescence. This technique enables high-quality 3D imaging for applications in pathology and lipid research.

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Area of Science:

  • Biotechnology
  • Neuroscience
  • Histology

Background:

  • Tissue clearing methods often compromise lipid structures for transparency.
  • Existing lipid-preserving methods struggle to balance transparency, fluorescence retention, and speed.

Purpose of the Study:

  • To develop a rapid, water-based tissue clearing method that preserves lipids and fluorescence.
  • To overcome limitations of current clearing techniques in lipid research and 3D imaging.

Main Methods:

  • Developed a novel water-based clearing solution (FLUID).
  • Evaluated lipid preservation, fluorescence stability (up to 60 days), and tissue morphology.
  • Tested compatibility with various fluorescent labels and pathological stains.

Main Results:

  • FLUID achieved rapid tissue clearing while preserving lipids and endogenous fluorescence.
  • Negligible tissue distortion was observed.
  • Demonstrated compatibility with diverse labeling techniques and pathological stains, including on human brain tissue.

Conclusions:

  • FLUID offers a balanced approach to tissue clearing, preserving lipids and fluorescence.
  • The method is suitable for high-quality 3D imaging, including applications in clinical pathology and biopsy.
  • FLUID advances 3D imaging capabilities for lipid research and diagnostics.