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Related Experiment Videos

Fluorescence identifies an alkaline cell in turtle urinary bladder.

M L Graber, T E Dixon, D Coachman

    The American Journal of Physiology
    |January 1, 1986
    PubMed
    Summary
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    Turtle bladder cells show distinct populations with varying intracellular pH (pHi). Carbonic anhydrase-rich cells appear more alkaline and play a unique role in acid secretion.

    Area of Science:

    • Cell Biology
    • Physiology
    • Biochemistry

    Background:

    • Intracellular pH (pHi) regulation is crucial for cellular function.
    • Turtle bladder mucosal cells exhibit heterogeneity in their physiological responses.

    Purpose of the Study:

    • To investigate the intracellular pH (pHi) of turtle bladder mucosal cells.
    • To identify distinct cell populations based on pHi and their role in acid secretion.

    Main Methods:

    • Utilized the trapped fluorescent indicator technique with 4-methylumbelliferone (4MU).
    • Employed pH-sensitive and pH-indifferent excitation wavelengths (365 nm and 334 nm) for quantitative pHi measurements.
    • Used specific cell markers like acridine orange and potential-sensing probes.

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    Main Results:

    • Identified 10-20% of mucosal cells with a more alkaline pHi (average difference of 0.1 pH units).
    • These alkaline cells showed distinct staining and morphology changes correlated with H+ secretion.
    • Carbonic anhydrase-rich cells were identified as the population with more alkaline pHi.

    Conclusions:

    • Acidification in turtle bladder cells involves H+ secretion via the luminal membrane.
    • Carbonic anhydrase supports base exit, with carbonic anhydrase-rich cells playing a unique role in H+ secretion.
    • Cellular morphology and pHi are dynamically regulated in response to acid secretion demands.