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Biplane ventriculography in the rat.

J L Heckman, L Garvin, T Brown

    The American Journal of Physiology
    |January 1, 1986
    PubMed
    Summary
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    High-speed biplane ventriculography accurately measures rat left ventricle dimensions and volumes. This technique provides reliable cardiac output and ejection fraction data in small animal models.

    Area of Science:

    • Cardiovascular physiology
    • Medical imaging technology

    Background:

    • Accurate assessment of cardiac function in small animal models is crucial for preclinical research.
    • Traditional methods for measuring left ventricular volumes and dimensions in rats can be challenging due to their small size.

    Purpose of the Study:

    • To evaluate the accuracy and feasibility of using high-speed biplane ventriculography for quantifying rat left ventricular (LV) dimensions and volumes.
    • To establish reference values for LV parameters in healthy anesthetized rats.

    Main Methods:

    • Biplane ventriculography was performed on nine anesthetized rats at high frame rates (500 frames/s) with direct enlargement.
    • Left ventricular silhouettes were digitized to calculate end-diastolic volume, end-systolic volume, stroke volume, ejection fraction, and cardiac output.

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  • Methyl methacrylate casts of 11 rat left ventricles were used to validate volume measurements against water displacement and monoplane/biplane methods.

    • Main Results:

    • Accurate LV dimensions and volumes were obtained, including end-diastolic volume (0.60 ± 0.03 ml) and ejection fraction (0.63 ± 0.02).
    • Cardiac output was measured at 118 ± 7 ml/min.
    • High correlation (r = 0.99 ± 0.02) was found between cast volumes and ventriculography measurements, confirming method accuracy.

    Conclusions:

    • High-speed biplane ventriculography is a validated and accurate method for assessing rat left ventricular function.
    • This technique provides reliable volumetric and dimensional data essential for cardiovascular research in rodent models.