Seasonal dynamics of bacterial composition and functions in biological treatment of coking wastewater

Seasonal dynamics of bacterial composition and functions were demonstrated for the biological fluidized-bed bioreactors combined in the anoxic/aerobic1/aerobic2 (AOO) coking wastewater (CWW) treatment sequences. The bacterial composition and functions in the CWW activated sludge samples were revealed by 16S rRNA genes amplicon sequencing. Thiobacillus, Cloacibacterium, Alkaliphilus and Pseudomonas were determined as core genera with seasonal changes. Mutable microbial community composition fluctuated in different seasons in same bioreactor. Distributions of predicted KEGG pathways along four seasons consistently demonstrated enrichment in biodegradation of carbon- and nitrogen-containing compounds. The major contaminants were removed from CWW by biochemical pathway of xenobiotics biodegradation and metabolism. This Level 2 pathway mainly owned the Level 3 pathways of benzoate degradation, drug metabolism-other enzymes, drug metabolism-cytochrome P450, metabolism of xenobiotics by cytochrome P450, and aminobenzoate degradation. The RDA results showed that dissolved oxygen with seasonal fluctuation was the main parameter shaping the microbial community. The observed dynamics within the microbial community composition, coupled with the maintained stability of CWW treatment efficiencies and a consistent profile of microbial functional pathways, underscore the presence of functional redundancy in the AOO bioreactors. The study underscored stable and effective operational performances of bioreactors in the AOO sequences, contributing the knowledge of microbiological basics to the advancement of CWW biological treatment. KEY POINTS: • Seasonal fluctuations of bacterial composition described for the AOO system. • Seasonal distributions of metabolic functions focused on carbon and nitrogen removal. • Functional redundancy was revealed in the AOO microbial community.