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Updated: May 5, 2026

An Aptamer-based Sensor for Unchelated GadoliniumIII
Published on: January 9, 2017
Construction of a reaction-based fluorescent sensor for tandem detection of Cu2+ and glutathione in wine
Han Yang1, Renqiang Chen1, Linjun Dai1
1College of Chemistry and Material Science, Sichuan Normal University, Chengdu, Sichuan 610066, China.
Abstract:
The purpose of this study was to develop a novel reaction-based fluorescent sensor for the detection of Cu2+ and glutathione in real wine samples. The sensor, tris-(2-pyridyl)-methylamine rhodol derivative, was synthesized and validated for the tandem and selective detection of both Cu2+ and glutathione. The sensor exhibited a strong linear correlation between fluorescence intensity and Cu2+ concentration ranging from 100 to 900 nM, while the in situ generated Cu2+ ensemble selectively detected glutathione with a robust linear response from 3 to 30 μM. The detection limits for Cu2+ and glutathione were as low as 28 nM and 0.60 μM, respectively. Additionally, the sensor enabled quantitative detection of Cu2+ and glutathione in real wine samples. This work provides the first reaction-based fluorescence sensor with an "on-off-on" fluorescence response for the tandem detection of Cu2+ and glutathione in wine, offering potential applications in food and beverage quality control.
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