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Related Experiment Videos

A Bacteroides by-product inhibits human polymorphonuclear leukocyte function.

O D Rotstein, T L Pruett, J J Sorenson

    Archives of Surgery (Chicago, Ill. : 1960)
    |January 1, 1986
    PubMed
    Summary

    Bacteroides species produce a heat-stable factor that inhibits human polymorphonuclear leukocyte (PMN) function. This inhibition may explain how Bacteroides enhances the pathogenicity of mixed bacterial infections.

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    Area of Science:

    • Microbiology
    • Immunology

    Background:

    • Bacteroides fragilis enhances Escherichia coli-induced lethality in animal models.
    • A potential mechanism involves B. fragilis inhibiting host defense mechanisms.

    Purpose of the Study:

    • To investigate the effect of Bacteroides species' culture filtrates on human polymorphonuclear leukocyte (PMN) function.
    • To identify factors contributing to the inhibition of neutrophil migration and function.

    Main Methods:

    • In vitro testing of culture filtrates from three Bacteroides species (B. fragilis, B. distasonis, B. thetaiotaomicron) on human PMN chemotaxis and random migration.
    • Analysis of factors influencing the inhibitory effect, including bacterial growth, culture pH, heat stability, molecular weight, and reversibility of PMN function inhibition.

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    Main Results:

    • Culture filtrates of B. fragilis and B. distasonis impaired PMN migration.
    • B. thetaiotaomicron showed variable inhibition of neutrophil chemotaxis.
    • The inhibitory factor was heat-stable, had a molecular weight <500, and its effect was partially irreversible.
    • Inhibition depended on adequate bacterial growth and reduced culture pH.

    Conclusions:

    • Bacteroides species produce a leukotoxic factor that inhibits PMN function.
    • This factor may contribute to the pathogenicity of mixed infections by impairing host neutrophil defenses.
    • The findings support the role of Bacteroides in modulating host immune responses during polymicrobial infections.