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Updated: Jun 10, 2025
05:22
Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
Published on: January 20, 2023
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Engineering quantitative stomatal trait variation and local adaptation potential by cis-regulatory editing.
Nicholas G Karavolias1,2, Dhruv Patel-Tupper2, Ana Gallegos Cruz1
1Innovative Genomics Institute, Berkeley, California, USA.
Plant Biotechnology Journal
|October 19, 2024
Summary
Cis-regulatory editing in rice created new stomatal density variations. This research links these changes to plant water use efficiency and drought response, offering a new way to optimize crop traits.
Area of Science:
- Plant genetics
- Molecular biology
- Crop science
Background:
- Coding sequence mutations can cause harmful effects.
- Cis-regulatory element editing offers an alternative for trait variation.
Purpose of the Study:
- To generate and analyze genotypic variation in the OsSTOMAGEN promoter using CRISPR/Cas9.
- To investigate the physiological and developmental consequences of this engineered variation.
Main Methods:
- Multiplexed CRISPR/Cas9 editing informed by bioinformatic data.
- Generation of genotypic variation in the OsSTOMAGEN promoter.
- Physiological assays under varying light and drought conditions.
Main Results:
- Engineered variation resulted in a 70%-120% range of wild-type stomatal density.
- Established relationships between stomatal morphology, conductance, carbon assimilation, and water use efficiency.
- Reduced drought-responsive stomatal density reprogramming in edited alleles.
Conclusions:
- Cis-regulatory editing effectively generates near-isogenic trait variation.
- This approach facilitates establishing anatomy-physiology relationships.
- Provides a foundation for optimizing plant traits for diverse environments.
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