Bioengineering of glucan coated silver nanoparticles as dynamic biomedical compound; in vitro and in vivo studies

The use of silver nanoparticles (AgNPs) gaining importance for the treatment of microbial infections and are in great demand due to their efficient broad antibacterial action but there is only one problem that silver nanoparticles can cause tissue damage. Therefore, the present study evaluated antimicrobial potential and intricacy of glucan coated silver nanoparticles in comparison with free silver nanoparticles. In this study, glucan coated silver nanoparticles (Glucan-AgNPs) by using Pleurotus spps. were characterized for their antimicrobial, minimum inhibitory concentration (MIC), biofilm inhibition, mutagenicity potential, hemolytic activities and histological examination through in vitro and in vivo analysis. The liver, kidney, intestine, and skin tissues were examined to gauge the adverse effects of the treatment method's toxicity by silver deposition. The results of this study have shown that mushroom's glucan extracted from Pleurotus spps. are excellent reducing agent and due to their best capping ability they reduce the toxicity of AgNPs and enhance their antimicrobial activities. The highest zone of inhibition was observed by Glucan-AgNPs from P. ostreatus (24 mm) against S. aureus while least zone of inhibition was resulted from Glucan-AgNPs from P. sapidus (14 mm) against B. subtilis. The results for biofilm inhibition showed excellent biofilm inhibition ability of Glucan-AgNPs. In results, maximum inhibition 95.2 % was observed by Glucan-AgNPs from P. ostreatus against S. aureus, while minimum inhibition 79.2 % by Glucan-AgNPs of P. sapidus against E. coli. Furthermore, Glucan-AgNPs treated mice showed no deposition and damage in the organs. Glucan-AgNPs has a higher efficacy in treating microbial infection.