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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 10, 2025

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Published on: January 9, 2019

Brief guide to flow cytometry.

Youngkwon Song¹, Yoontae Lee¹

¹Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk 37673, Republic of Korea.

Molecules and Cells

|October 19, 2024

Summary

This summary is machine-generated.

Flow cytometry is a versatile cell analysis technique. This guide details its principles and practical application, from setup to data analysis, aiding researchers in its effective use.

Keywords:

Compensation Flow cytometry Fluorophore Gating strategy Panel design

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Area of Science:

Biotechnology
Cell Biology
Immunology

Background:

Flow cytometry is a crucial analytical method.
It measures physical and chemical properties of cells and particles.
Its applications span diverse research fields.

Purpose of the Study:

To provide a concise guide to flow cytometry principles.
To detail the utilization of flow cytometry in research.
To offer step-by-step instructions for instrument setup and data analysis.

Main Methods:

Principles of fluid stream and light beam interaction.
Instrument setup procedures.
Data acquisition and analysis techniques.

Main Results:

A comprehensive overview of flow cytometry.
Practical guidance for researchers.
Facilitation of successful application in studies.

Conclusions:

Flow cytometry is a powerful tool for cell analysis.
This guide empowers researchers to effectively utilize the technique.
Successful implementation enhances scientific discovery.