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Related Experiment Video

Updated: Jun 10, 2025

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Hippocampal recording with a soft microelectrode array in a cranial window imaging scheme: a validation study.

G Juhász1, M Madarász2,3, B Szmola2,1

  • 1Two-Photon Measurement Technology Research Group, The Faculty of Information Technology, Pázmány Péter Catholic University, Budapest, Hungary.

Scientific Reports
|October 19, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a transparent microelectrode device for simultaneous two-photon imaging and electrophysiology in mouse hippocampus. The device enables long-term recordings of neural activity and cell imaging for over six months.

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Optical Imaging

Background:

  • The hippocampus is vital for memory and navigation.
  • Two-photon imaging and electrophysiology are key neuroscience tools.
  • Chronic in vivo recordings are essential for understanding long-term neural processes.

Purpose of the Study:

  • To develop and validate a novel transparent microelectrode device for combined in vivo imaging and electrophysiology.
  • To assess the long-term stability and performance of the device in awake, behaving mice.
  • To investigate the compatibility of the device with chronic calcium imaging and electrophysiological recordings.

Main Methods:

  • High-speed two-photon hippocampal imaging in awake mice using a soft, transparent microelectrode (STM) device.
  • Chronic implantation of the STM device within a cranial window chamber.
  • Simultaneous recording of local field potentials (LFPs), multi-unit activity (MUA), and single-unit activity (SUA).
  • Monitoring of electrode impedance for long-term recording quality assessment.
  • Calcium imaging of GCaMP6f-labeled cells in the CA1 pyramidal layer.
  • Post-experiment histological analysis using GFAP staining to assess immune response.

Main Results:

  • The STM device enabled reliable, large-scale electrophysiological recordings (LFP, MUA, SUA) from the dorsal hippocampus for up to two months.
  • High-quality calcium imaging of GCaMP6f-labeled cells was achieved for over six months in thy1-GCaMP6f transgenic mice.
  • Electrode impedance remained stable, indicating good long-term recording quality.
  • Histological analysis showed minimal immune response after long-term implantation.

Conclusions:

  • The developed transparent microelectrode device is suitable for simultaneous, long-term two-photon imaging and large-scale electrophysiological measurements in chronic mouse models.
  • This technology offers a valuable tool for advancing neuroscience research, particularly in memory and navigation studies.
  • The device demonstrates excellent biocompatibility and stability for extended in vivo experiments.