Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

876
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
876
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

315
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
315
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.0K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.0K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

297
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
297
¹H NMR of Labile Protons: Temporal Resolution01:10

¹H NMR of Labile Protons: Temporal Resolution

1.1K
Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
1.1K
IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

5.3K
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metal Oxide Nanocomposites as Next-Generation Antimicrobial Agents Against Oral Cariogenic Pathogens: Mechanistic Actions of Ag-ZnO and Cu-ZnO on <i>S. mutans</i> and <i>S. sobrinus</i>.

Materials (Basel, Switzerland)·2026
Same author

Eutectic Coamorphous System of Enzalutamide and Acetyl Maltose: A Strategy for Improved Physical Stability and Aqueous Solubility.

Molecular pharmaceutics·2026
Same author

From trace to trace maker: Oligocene-Miocene coprolites of southern Poland and their potential producers.

PeerJ·2025
Same author

Novel pyrido[2,3-d]pyrimidines for bioimaging: Effect of water content on enhanced fluorescence and the mechanism of aggregation-induced blue-shifted emission.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025
Same author

Changes of structural, magnetic and spectroscopic properties of microencapsulated iron sucrose nanoparticles in saline.

Beilstein journal of nanotechnology·2025
Same author

Tuning Ionic Liquids with Charged Polyhedral Oligomeric Silsesquioxane Nanoparticles for Highly Conductive Quasi-Solid Electrolytes.

Nano letters·2025

Related Experiment Video

Updated: Jun 10, 2025

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
07:52

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Published on: April 12, 2017

12.8K

Hydroxyl group dynamics in defernite: Raman spectroscopy studies.

Dorota Środek1, Mateusz Dulski2

  • 1University of Silesia, Faculty of Natural Sciences, Będzińska 60, Sosnowiec, Poland.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|October 20, 2024
PubMed
Summary

A rare mineral, defernite, was discovered in the Upper Chegem Caldera. Raman spectroscopy revealed its unique structural properties, including hydrogen bonding and hydroxyl group characteristics.

Keywords:
DeferniteHydrogen bondsPyrometamorphismRaman spectroscopy

More Related Videos

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
07:53

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

Published on: March 1, 2020

7.2K
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

9.8K

Related Experiment Videos

Last Updated: Jun 10, 2025

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
07:52

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Published on: April 12, 2017

12.8K
Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
07:53

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

Published on: March 1, 2020

7.2K
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

9.8K

Area of Science:

  • Mineralogy
  • Geochemistry
  • Spectroscopy

Background:

  • Silicate-carbonate xenoliths provide insights into geological processes.
  • Rare mineral occurrences expand our understanding of Earth's composition.
  • The Upper Chegem Caldera is a site of significant geological interest.

Purpose of the Study:

  • To identify and characterize a rare mineral, defernite, found in the Upper Chegem Caldera.
  • To perform a detailed structural analysis of defernite using Raman spectroscopy.
  • To compare the spectral properties of defernite with known mineral samples.

Main Methods:

  • Detailed examination of altered silicate-carbonate xenoliths.
  • Raman spectroscopy for structural analysis.
  • Polarization-dependent and temperature-dependent experiments.

Main Results:

  • Discovery of defernite (Ca 6[(CO 3) 2-x(Si 2O 7) x/2](OH) 7[Cl 1-x(H 2O) x], x ≈ 0.4) as colorless to white fibrous aggregates.
  • Raman spectra showed characteristic bands for carbonate ions (1085 cm -1) and hydroxyl groups (3590-3600 cm -1).
  • Observed changes in band intensities under altered laser polarization and temperature, indicating hydrogen bonding and hydroxyl group behavior.

Conclusions:

  • Defernite represents a new occurrence of this rare carbonate mineral.
  • Raman spectroscopy effectively elucidates defernite's structural features, including hydrogen bonding.
  • The study provides key insights into the vibrational properties and structural stability of defernite.