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Related Experiment Video

Updated: Jun 10, 2025

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
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Mitoquinone improves porcine embryo development through modulating oxidative stress and mitochondrial function.

Dabin Cha1, Seunghyun Choi1, Yumin Lee1

  • 1Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon, 34134, Republic of Korea.

Theriogenology
|October 20, 2024
PubMed
Summary

Mitoquinone (MitoQ) supplementation enhances porcine embryo development by reducing oxidative stress and improving mitochondrial function during in vitro culture. The optimal concentration of 0.1 nM MitoQ boosts blastocyst formation and cell quality.

Keywords:
Developmental competenceIn vitro embryo productionMitochondrial-targeted antioxidantMitoquinonePorcine embryo

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Oxidative stress from reactive oxygen species (ROS) impairs in vitro embryo production.
  • ROS cause mitochondrial dysfunction and apoptosis, hindering embryo development.

Purpose of the Study:

  • To investigate the effects of mitoquinone (MitoQ), a mitochondrial-targeted antioxidant, on porcine embryo development during in vitro culture (IVC).
  • To determine the optimal concentration of MitoQ for improving porcine IVC efficiency.

Main Methods:

  • Porcine embryos were cultured in vitro with varying concentrations of MitoQ (0, 0.01, 0.1, or 1 nM).
  • Evaluated blastocyst formation rate, cell numbers, apoptosis, and gene expression related to lineage, apoptosis, and antioxidants.
  • Assessed intracellular ROS, glutathione levels, mitochondrial quantity, membrane potential, and ATP content.

Main Results:

  • 0.1 nM MitoQ significantly increased blastocyst formation rate and total cell number.
  • MitoQ treatment reduced apoptotic cells and upregulated apoptosis-related gene expression.
  • MitoQ decreased ROS, increased glutathione, and enhanced mitochondrial function (quantity, membrane potential, ATP) and biogenesis gene expression.

Conclusions:

  • Mitoquinone (MitoQ) at 0.1 nM is optimal for porcine IVC.
  • MitoQ enhances porcine embryo developmental competence by mitigating oxidative stress and improving mitochondrial function.