Tolerance of Pseudomonas oleovorans biofilms to disinfectants commonly used in endoscope reprocessing?
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View abstract on PubMed
Summary
This summary is machine-generated.Persistent growth of Pseudomonas oleovorans (PSOL) in endoscope disinfection indicates reprocessing failures. Higher disinfectant concentrations may eradicate PSOL, but require further study to avoid instrument damage and patient harm.
Area Of Science
- Microbiology
- Infectious Diseases
- Healthcare-Associated Infections
Background
- Reprocessing failures in automated endoscope washer-disinfectors (AEWDs) can lead to Gram-negative bacterial infections in patients.
- Pseudomonas oleovorans (PSOL) was identified during routine quality control checks in AEWDs and on endoscopes.
Purpose Of The Study
- To investigate the efficacy of a disinfectant (GLUT) against planktonic and biofilm forms of PSOL under conditions simulating AEWD disinfection processes.
- To assess the implications of PSOL persistence for endoscope reprocessing quality control.
Main Methods
- Genotyping was used to identify two different PSOL strains.
- Planktonic and biofilm cultures of PSOL and Pseudomonas aeruginosa PAO1 were exposed to varying concentrations of GLUT at different temperatures.
- Disinfection efficacy was evaluated by monitoring bacterial eradication.
Main Results
- PSOL in planktonic form was eradicated by 1% GLUT at 55°C.
- 3% GLUT eradicated PSOL biofilms at 25°C.
- The continued growth of PSOL in quality controls suggests inadequate disinfection protocols.
Conclusions
- Inadequate endoscope disinfection can allow persistent growth of bacteria like PSOL.
- Increasing GLUT concentration could be a strategy to eliminate PSOL, but potential instrument corrosion and patient toxicity need consideration.
- Further research is needed on disinfectant properties, contamination sources, and additional disinfection steps to ensure patient safety.
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