Differential inhibition of tire wear particles on sludge dewatering by aging modes

The study assessed the acute toxicities of tire wear particles (TWPs) on activated sludge, comparing cryogenically ground TWPs (C-TWPs) with photo-aged (PA-TWPs), ozone-aged (OA-TWPs), and Fenton-aged (FA-TWPs) variants over 96 h. At 0.1 mg/L, TWPs showed no significant effects on sludge respiration or purification. However, at 50 mg/L, significant impacts on respiration, decontamination capacity, and microbial community structure were observed, particularly in aged TWPs. Specifically, aged TWPs, especially FA-TWPs, are prone to inducing necrosis by generating non-cellular reactive oxygen species (ROS) catalyzed by persistent free radicals, leading to an increase in lactate dehydrogenase release ranging from 215 % to 284 %. Conversely, C-TWPs tend to trigger apoptosis via intracellular ROS accumulation, leading to a 358 % increase in intracellular ROS. Aged TWPs exhibited higher affinities for proteins and polysaccharides, while C-TWPs preferred phospholipids. All TWPs adversely affected sludge dewatering, with strong correlations found between specific resistance to filtration (SRF) and total protein (r = 0.981, p < 0.001) and between bound water and early cell apoptosis (r = 0.961, p < 0.01). Additionally, a correlation between SRF and cellular necrosis (r = 0.956, p < 0.01) was noted, linked to increased protein and extracellular polymeric substance levels. These results emphasize substantial influence of aged TWPs on sludge dewatering efficiency via diverse bacterial cell death mechanisms.