Temperature dependence of lipogenesis in isolated hepatocytes from rainbow trout (Salmo gairdneri)

Temperature dependence of lipogenesis in trout liver cells was investigated in the presence of 5 mM lactate using either [14C]lactate or [3H]water. A ratio of 3H/14C-incorporation greater than one is found, irrespective of temperature. Acclimation of fish to 4, 10 or 16 degrees C affects neither the height of lipid synthesis nor its temperature sensitivity. The distribution of [14C]lactate between the main lipid classes and the capacities for cholesterol- and triacylglycerol-synthesis are correlated to the glycogen stores of the hepatocytes. A comparison of fatty acid synthesis and cholesterogenesis in livers of normal fed rat and of trout suggests a capability for lipogenesis in trout somewhat similar to that in mammals.