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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

The Antiviral System of Bacteria and Archaea: CRISPR

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.

Smallpox

Smallpox

Smallpox is a severe contagious disease caused by the Variola major virus, a double-stranded DNA member of the Poxviridae family.Variola major transmission occurs primarily via inhalation of virus-laden droplets or direct contact with infectious scabs. The incubation period averages approximately seven days, although it may range from 7 to 17 days depending on the inoculum and host factors.Clinically, the prodromal phase is marked by an abrupt onset of high fever, malaise, headache, and myalgia.

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Related Experiment Video

Updated: Jun 23, 2026

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

CRISPR-based strategies for sample-to-answer monkeypox detection: current status and emerging opportunities.

Md Ahasan Ahamed¹, Anthony J Politza², Tianyi Liu¹

¹Department of Electrical Engineering, Pennsylvania State University, University Park, PA 16802, United States of America.

|October 21, 2024

Summary

Rapid Monkeypox virus (Mpox) detection is crucial. Clustered regularly interspaced short palindromic repeats (CRISPR)-based point-of-care devices offer sensitive, specific, and programmable solutions for decentralized diagnostics and tracking viral mutations.

Keywords:

CRISPR Cas12 RPA monkeypox nucleic acid point-of-care

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

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Published on: April 25, 2025

Area of Science:

Molecular Biology
Virology
Biotechnology

Background:

The global Monkeypox virus (Mpox) outbreak necessitates rapid, accessible diagnostic tools.
Decentralized point-of-care (POC) testing is vital for effective Mpox management.
Clustered regularly interspaced short palindromic repeats (CRISPR) technology shows promise for nucleic acid detection.

Purpose of the Study:

To review recent advancements in CRISPR-based POC devices for Mpox detection.
To highlight the role of genetic sequencing in Mpox diagnostics and surveillance.
To explore the integration of CRISPR-Cas12 systems with amplification techniques.

Main Methods:

Review of current literature on CRISPR-based Mpox detection strategies.
Analysis of CRISPR-Cas12 systems, including crRNA design and Cas12 protein function.
Investigation of combined CRISPR-Cas12 with pre-amplification and amplification-free methods, including recombinase polymerase amplification (RPA).

Main Results:

CRISPR technology offers high sensitivity and specificity for nucleic acid detection.
CRISPR-Cas12 coupled with RPA demonstrates effectiveness for Mpox detection.
Genetic sequencing is essential for understanding Mpox evolution and designing diagnostic tools.

Conclusions:

CRISPR-Cas12-based POC devices are a promising next-generation molecular biosensing technology.
These devices can facilitate rapid, decentralized detection of Mpox.
Further development is needed to address future prospects and challenges in Mpox diagnostics.