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Genomic Characterization and Probiotic Properties of Lactiplantibacillus pentosus Isolated from Fermented Rice
Athira Cheruvari1,2, Rajagopal Kammara3,4
1Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, 570020, India.
Probiotics and Antimicrobial Proteins
|October 21, 2024
Summary
This study characterized Lactiplantibacillus pentosus (krglsrbmofpi2) from fermented rice, revealing probiotic potential. The strain exhibits strong antagonistic activity against major pathogens, suggesting food industry applications.
Area of Science:
- Microbiology
- Genomics
- Food Science
Background:
- Traditionally fermented foods are sources of beneficial microorganisms.
- Lactiplantibacillus pentosus is a lactic acid bacterium with known probiotic properties.
- Characterization of novel strains is crucial for identifying new probiotics.
Purpose of the Study:
- To perform preliminary genetic and phenotypic characterization of a Lactiplantibacillus pentosus strain (krglsrbmofpi2) from fermented rice.
- To assess the safety and probiotic potential of the identified strain.
- To evaluate its antagonistic activity against priority pathogens.
Main Methods:
- Whole genome sequencing and bioinformatic analysis.
- Biochemical and biophysical analyses (auto-aggregation, co-aggregation, hydrophobicity, antioxidant activity, gastrointestinal tolerance).
- Safety evaluation through hemolytic activity testing.
- Antimicrobial activity testing against WHO priority pathogens.
Main Results:
- The L. pentosus strain krglsrbmofpi2 possesses a 3.7-Mb genome with 3192 protein-coding sequences.
- Bioinformatic analysis identified genes related to phages, plasmids, pathogenicity, antibiotic resistance, and bacteriocins.
- The strain demonstrated significant auto-aggregation, co-aggregation, antioxidant activity, and tolerance to simulated gastrointestinal conditions.
- It exhibited strong antagonistic activity against Vibrio cholerae, Clostridium perfringens, Salmonella Typhi, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus.
- No hemolytic activity was observed, indicating safety.
Conclusions:
- Lactiplantibacillus pentosus strain krglsrbmofpi2 shows promising probiotic characteristics, including broad-spectrum antimicrobial activity.
- Its genetic and functional properties warrant further investigation for potential use as a probiotic in the food industry.
- This strain represents a potential candidate for developing functional foods and probiotics.
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