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Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents
Published on: February 15, 2022
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Mouse Model of Glucocorticoid-Induced Glaucoma
Prabhavathi Maddineni1,2, Yogapriya Sundaresan1, Gulab Zode3
1Gavin Herbert Eye Institute-Center for Translational Vision Research, Department of Ophthalmology, Department of Physiology and Biophysics, University of California Irvine School of Medicine, Irvine, California, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2024
Summary
Glucocorticoid (GC) eye drops can cause glaucoma. This study details a mouse model using dexamethasone acetate to mimic human POAG, aiding glaucoma research and therapy development.
Area of Science:
- Ophthalmology
- Pharmacology
- Neuroscience
Background:
- Extended glucocorticoid (GC) treatment can cause ocular hypertension and iatrogenic open-angle glaucoma.
- Glucocorticoid-induced glaucoma shares features with primary open-angle glaucoma (POAG), necessitating reliable animal models for study.
Purpose of the Study:
- To present a detailed protocol for a mouse model of glucocorticoid-induced glaucoma.
- To establish a model that replicates key pathological features of human POAG for research.
Main Methods:
- Weekly periocular injections of dexamethasone-21-acetate (Dex-Ac) in mice.
- Assessment of intraocular pressure (IOP), retinal ganglion cell (RGC) loss, and optic nerve degeneration.
- Analysis of trabecular meshwork (TM) function, extracellular matrix deposition, and endoplasmic reticulum stress.
Main Results:
- Dex-Ac injections induced significant IOP elevation and RGC loss.
- The model demonstrated reduced aqueous outflow facility and TM dysfunction, characteristic of POAG.
- Progressive optic nerve degeneration was observed, mirroring glaucomatous damage.
Conclusions:
- The dexamethasone-induced glaucoma mouse model accurately mimics human POAG.
- This model is suitable for investigating TM and RGC axon damage in glaucoma.
- It serves as a valuable platform for developing novel glaucoma therapies.
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