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Related Experiment Video

Updated: Jun 9, 2025

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice
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A Comprehensive Protocol for Microbead-Induced Ocular Hypertension in Mice.

M Reza Bahrani Fard1, Nina Sara Fraticelli-Guzmán1, Jessica Chan2

  • 1George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2024
PubMed
Summary

This study refines the microbead occlusion model for inducing ocular hypertension in mice, a key glaucoma model. The updated protocol enhances consistency for reliable preclinical glaucoma research.

Keywords:
GlaucomaIntraocular pressureMicrobeadMouse modelOcular hypertensionRodent

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Area of Science:

  • Ophthalmology
  • Neuroscience
  • Animal Models

Background:

  • Glaucoma involves retinal ganglion cell (RGC) degeneration.
  • Elevated intraocular pressure (IOP) is a primary risk factor and characteristic of glaucoma models.
  • Laboratory mice offer genetic diversity for preclinical glaucoma research.

Purpose of the Study:

  • To provide an updated, comprehensive protocol for the microbead occlusion model.
  • To improve procedural consistency and outcome measure reliability.
  • To enable single-experimenter execution of the model.

Main Methods:

  • Detailed protocol for the microbead occlusion model in mice.
  • Focus on surgical techniques and outcome measurement consistency.
  • Adaptations to enhance IOP elevation magnitude and duration.

Main Results:

  • An updated protocol for consistent microbead model execution.
  • Improved reliability in surgical procedures and outcome assessments.
  • Facilitation of single-experimenter operation.

Conclusions:

  • The refined microbead occlusion protocol enhances consistency and accessibility.
  • This standardized model supports more reliable preclinical glaucoma research.
  • The updated protocol aids in studying ocular hypertension and RGC degeneration.