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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Related Experiment Video

Updated: Jun 9, 2025

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
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Spatial-transcriptomic profiling: a new lens for understanding myelofibrosis pathophysiology.

Edoardo Peroni1, Elisabetta Calistri2, Rosario Amato3,4

  • 1Immunology and Molecular Oncology Unit, Veneto Institute of Oncology, IOV-IRCCS, Padova, 35128, Italy. edoardo.peroni@iov.veneto.it.

Cell Communication and Signaling : CCS
|October 21, 2024
PubMed
Summary

Myelofibrosis (MF) research is advanced by Spatially Resolved Transcriptomics (SRT), offering new insights into bone marrow fibrosis and spleen pathology. This technology aids in identifying novel therapeutic targets and improving diagnostics for MF patients.

Keywords:
Jak inhibitorsMyelofibrosisMyeloproliferative neoplasmsPersonalized medicineSpatial transcriptomicsSpleen

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Area of Science:

  • Hematology
  • Genomics
  • Oncology

Background:

  • Myelofibrosis (MF) is a myeloproliferative neoplasm marked by bone marrow fibrosis and abnormal stem cell proliferation.
  • Driver mutations (JAK2, CALR, MPL) and additional mutations (TET2, SRSF2, TP53) contribute to MF complexity and JAK-STAT pathway activation.
  • The bone marrow microenvironment, including stromal cells and cytokines, is crucial in MF pathogenesis.

Purpose of the Study:

  • To explore the utility of Spatially Resolved Transcriptomics (SRT) in understanding Myelofibrosis pathophysiology.
  • To investigate cellular heterogeneity, spatial gene regulation, and microenvironmental interactions in MF.
  • To identify novel therapeutic targets and biomarkers for MF through high-resolution spatial profiling.

Main Methods:

  • Application of Spatially Resolved Transcriptomics (SRT) for high-resolution gene expression mapping in bone marrow and spleen.
  • Profiling diverse cell populations and molecular alterations within the affected tissues.
  • Analysis of stromal-hematopoietic dynamics and microenvironmental interactions.

Main Results:

  • SRT provides unprecedented insights into spatial gene regulation and microenvironmental interactions in MF.
  • Revealed molecular signatures and pathological niches driving MF progression in the bone marrow and spleen.
  • Highlighted the spleen's role as a site of abnormal hematopoiesis and fibrotic changes in MF.

Conclusions:

  • SRT significantly enhances the understanding of MF pathophysiology by detailing spatial heterogeneity.
  • This technology facilitates the identification of novel therapeutic targets and biomarkers.
  • Integrating SRT into MF research promises improved diagnostic precision and therapeutic innovation.