Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.1K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.1K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

14.6K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
14.6K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

3.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.6K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.5K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.5K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

6.0K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.0K
Structure of Porins01:21

Structure of Porins

3.0K
Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a  motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interpretable prediction and generation of ASC-speck aptamers using multiscale deep biological learning models.

Bioinformatics advances·2026
Same author

Graph-based RNA structural representation reveals determinants of subcellular localization.

Briefings in bioinformatics·2026
Same author

Pueraria lobata extract mitigates diabetic nephropathy via NLRP3 inflammasome and Caspase-1 regulation.

Scientific reports·2026
Same author

RPI-PLMGNN: Enhancing RNA-Protein Interaction Prediction with the Pretrained Large Language Models and Graph Neural Networks.

ACS synthetic biology·2026
Same author

MPMFMol: Multitask Self-Supervised Pretraining with Multimodal Fine-Tuning for Molecular Property Prediction.

Journal of chemical information and modeling·2026
Same author

Quantum computing applications in drug discovery.

Briefings in bioinformatics·2026

Related Experiment Video

Updated: Apr 30, 2026

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
05:47

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

5.1K

A two-task predictor for discovering phase separation proteins and their undergoing mechanism.

Yetong Zhou1, Shengming Zhou2,3, Yue Bi4

  • 1School of Science, Dalian Maritime University, 1 Linghai Road, Dalian, 116026, China.

Briefings in Bioinformatics
|October 22, 2024
PubMed
Summary

Researchers developed Opt_PredLLPS, a computational tool to identify proteins driving liquid-liquid phase separation (LLPS) and their interaction mechanisms. This aids understanding of biomolecular condensates and cellular organization.

Keywords:
deep learningpartner-dependentphase separationself-assemblysequence analysis

More Related Videos

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.1K
A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

2.4K

Related Experiment Videos

Last Updated: Apr 30, 2026

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
05:47

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

5.1K
Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.1K
A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

2.4K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Computational Biology

Background:

  • Liquid-liquid phase separation (LLPS) is a key cellular mechanism for organizing biomolecules into membraneless organelles.
  • Identifying proteins that undergo LLPS is essential for understanding cellular compartmentalization and function.

Purpose of the Study:

  • To develop a computational tool, Opt_PredLLPS, for identifying potential phase separation proteins.
  • To predict the interaction-dependent mechanism of LLPS for identified proteins.

Main Methods:

  • A two-task deep learning model combining CNN and BiLSTM for initial LLPS protein prediction.
  • An XGBoost model with feature selection for predicting interaction-dependent LLPS mechanisms.
  • In silico saturation mutagenesis to identify critical phase separation regions.

Main Results:

  • Opt_PredLLPS effectively identifies potential LLPS proteins and differentiates their mechanisms.
  • The study validated the model's performance on benchmark datasets.
  • Key protein regions involved in phase separation were identified.

Conclusions:

  • Opt_PredLLPS provides a valuable resource for discovering phase separation proteins.
  • The findings advance the understanding of LLPS mechanisms and cellular organization.
  • This tool can guide future research in biomolecular condensates.