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  11. Histological And Photoacoustic Evaluation Of Rectal Cancer After Neoadjuvant Therapy Using Microvascular Density

Histological and photoacoustic evaluation of rectal cancer after neoadjuvant therapy using microvascular density

Ahmed A Eltahir1, Haolin Nie2, Sitai Kou2

  • 1Department of Surgery, Washington University School of Medicine, St Louis, Missouri, USA.

Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland
|October 22, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Microvascular density (MVD) in rectal cancer correlates with treatment response. Complete responders show higher MVD, potentially explaining photoacoustic imaging findings for non-operative management selection.

Area of Science:

  • Oncology
  • Medical Imaging
  • Pathology

Background:

  • Non-operative management of rectal cancer requires accurate staging and surveillance.
  • Transrectal acoustic resolution photoacoustic microscopy (ARPAM) shows promise in differentiating treatment responses.
  • Previous studies suggest ARPAM findings correlate with microvascular density (MVD).

Purpose of the Study:

  • To investigate the relationship between microvascular density (MVD) and treatment response in rectal cancer.
  • To determine if MVD changes explain findings from transrectal photoacoustic microscopy.
  • To validate ERG nuclei density as a proxy for MVD in post-neoadjuvant rectal cancer tissue.

Main Methods:

  • Compared CD31 and ERG immunostaining for endothelial identification in rectal cancer tissue.
Keywords:
ERGmicrovascular densityphotoacousticrectal cancer

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  • Correlated manual CD31 vessel counts with ERG nuclei density.
  • Quantified ERG nuclei density in complete responders, partial responders, and non-responders after neoadjuvant therapy.

    • Main Results:

    • CD31 MVD and ERG nuclei density showed a strong correlation (R² = 0.87, P < 0.01).
    • Complete responders exhibited significantly higher MVD (599 nuclei/mm²) compared to partial (185 nuclei/mm²) and non-responders (117 nuclei/mm²).
    • No significant MVD difference was observed between partial and non-responders.

    Conclusions:

    • Microvascular density is highest in complete responders to neoadjuvant therapy, resembling normal rectal tissue.
    • Histological microvascular patterns correlate with imaging patterns observed in photoacoustic microscopy.
    • MVD assessment may enhance the interpretation of imaging for rectal cancer surveillance and organ preservation selection.