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A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissues.

Sara C Neves1,2,3,4, Aureliana Sousa1,2, Diana S Nascimento1,2,5

  • 1i3S - Instituto de Investigação e Inovação em Saúde, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal.

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|October 22, 2024
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Summary

This study developed a hybrid 3D scaffold for tissue engineering. Optimized cell seeding created aligned microvascular networks, showing promise for functional tissue regeneration.

Keywords:
Cell contact guidanceRegenerative medicineScaffold vascularizationTherapeutic vascularizationVascularized tissue

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Functional microvascular networks are essential for engineered tissues to integrate with host vasculature.
  • Hybrid 3D scaffolds offer advanced strategies for tissue engineering applications.

Purpose of the Study:

  • To develop and evaluate a hybrid 3D scaffold system for guided microvascular network formation.
  • To optimize cell seeding protocols for enhanced microvessel development within the scaffold.

Main Methods:

  • Fabrication of a hybrid 3D scaffold combining poly(ethylene oxide terephthalate)/poly(butylene terephthalate) fibers and pectin hydrogels.
  • Sequential seeding of human endothelial cells (EC) and mesenchymal stromal cells (MSC) onto the scaffold.
  • In vitro culture under pro-angiogenic and osteoinductive conditions, followed by in vivo subcutaneous implantation in mice.

Main Results:

  • An optimized seeding strategy (EC on scaffold, MSC in hydrogel) promoted highly oriented, lumenized microvascular networks.
  • These networks were supported by basement membranes and pericyte-like cells, persisting for at least 28 days in vitro.
  • Constructs showed host vessel infiltration and retained human microvessels for 2 weeks post-implantation, with successful MSC osteogenic differentiation.

Conclusions:

  • The hybrid 3D system and optimized cell seeding effectively direct the formation of robust, geometrically oriented microvessels.
  • This approach holds significant promise for creating pre-vascularized tissue constructs for regenerative medicine.