Related Experiment Video
Updated: Jun 9, 2025
04:43
Visualizing Visual Adaptation
Published on: April 24, 2017
8.9K
Eleven shades of PASEF
Marta L Mendes1, Klara F Borrmann1,2, Gunnar Dittmar1,2
1Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg.
Expert Review of Proteomics
|October 22, 2024
Summary
Trapped ion mobility spectrometry (TIMS) coupled with mass spectrometry, particularly using Parallel Accumulation-Serial Fragmentation (PASEF), significantly enhances protein identification and quantification in proteomics. This technology is advancing clinical proteomics applications.
Area of Science:
- Proteomics
- Analytical Chemistry
- Biotechnology
Background:
- Trapped ion mobility spectrometry (TIMS) combined with fast high-resolution time-of-flight (TOF) mass spectrometry has revolutionized proteomics.
- Parallel Accumulation-Serial Fragmentation (PASEF) is a key technology driving advancements in protein identification and quantification.
- PASEF has been adapted for both discovery and targeted proteomics applications.
Purpose of the Study:
- To review the evolution and current state of PASEF measurement techniques.
- To highlight the application of these techniques in clinical proteomics.
- To discuss the impact of dual TIMS tunnels on proteomics depth and speed.
Main Methods:
- Literature search using PubMed and Google Scholar.
- Review of eleven newly implemented PASEF measurement techniques over the last decade.
- Analysis of PASEF applications in clinical proteomics.
Main Results:
- PASEF technology has led to a significant increase in protein identifications and quantifications.
- The limit of detection for proteins in biological samples has been lowered.
- Dual TIMS tunnels have enhanced the depth and speed of proteomics measurements.
Conclusions:
- PASEF techniques have continuously been optimized, leading to eleven new measurement methods.
- These advancements are significantly pushing the field of clinical proteomics forward.
- The dual TIMS tunnel approach is revolutionizing proteomics analysis.
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