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Development of bacterial bioformulations using response surface methodology.

Priyanka1, Shashi Kumar1, Shilpi Sharma1

  • 1Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110016, India.

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|October 22, 2024
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Summary
This summary is machine-generated.

Developing a stable bioformulation using bacterial mutants enhances Cajanus cajan growth. This sustainable approach improves crop yield by ensuring bacterial viability in soil conditions.

Keywords:
bacterial consortiabioformulationplant growth promotionresponse surface methodology

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Area of Science:

  • Agricultural Microbiology
  • Plant Science
  • Biotechnology

Background:

  • Bacterial consortia offer sustainable crop improvement but face viability challenges.
  • Developing robust bioformulations is crucial for their field application.

Purpose of the Study:

  • To develop an efficient bioformulation for plant growth-promoting bacteria.
  • To enhance the viability and performance of bacterial inoculants for Cajanus cajan.

Main Methods:

  • Optimized solid and liquid bioformulations using carboxymethylcellulose (CMC), glycerol, polysorbate, and polyvinyl pyrrolidone (PVP).
  • Assessed bioinoculant stability at 4°C and 30°C.
  • Evaluated the in planta efficiency of the triple culture consortium in sterile and non-sterile soil.

Main Results:

  • Achieved high cell counts in optimized solid (e.g., 8.10 × 10^8 CFU g^-1 Priestia megaterium) and liquid formulations (e.g., 8 × 10^9 CFU ml^-1 P. megaterium).
  • Demonstrated superior bioinoculant viability (6 months) at 4°C compared to 30°C.
  • Observed enhanced Cajanus cajan growth with the triple culture consortium, with strains detectable in soil for 45 days.

Conclusions:

  • A systematic process for creating potent bioformulations was established.
  • Mutant strains facilitated tracking of bioinoculants.
  • The triple culture consortium significantly outperformed mono and dual cultures in promoting plant growth.