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Related Experiment Videos

Trypsin in human milk.

J C Monti, A F Mermoud, P Jollès

    Experientia
    |January 15, 1986
    PubMed
    Summary

    Human milk trypsin, a key enzyme, was purified and its concentration measured. This research provides foundational data on human milk trypsin levels.

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    Area of Science:

    • Biochemistry
    • Human Milk Analysis
    • Enzymology

    Background:

    • Human milk contains various enzymes crucial for infant nutrition.
    • Trypsin is a protease found in human milk, but its properties and concentration are not fully characterized.

    Purpose of the Study:

    • To purify human milk trypsin.
    • To determine the molecular weight and concentration of human milk trypsin.

    Main Methods:

    • Purification using adsorption chromatography on cellulose-bound 4-aminobenzamidine.
    • Molecular weight determination.
    • Concentration measurement via radioimmunoassay.

    Main Results:

    • Human milk trypsin was successfully purified.
    • The molecular weight of human milk trypsin was determined to be approximately 24,000 daltons.
    • The concentration of human milk trypsin was found to range from 2.9 to 5.6 micrograms/l.

    Conclusions:

    • Human milk trypsin is a distinct enzyme with a specific molecular weight.
    • The quantifiable concentration of human milk trypsin provides baseline data for further research.