Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatial structures in mitochondrial suspension induced by cation efflux.

E L Holmuhamedov, Evtodienko YuV

    FEBS Letters
    |January 20, 1986
    PubMed
    Summary

    Spatial structure formation in mitochondria depends on ion transport. Pattern development is linked to cation efflux and requires energy, highlighting the role of divalent cations in this process.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Adaphostin and other anticancer drugs quench the fluorescence of mitochondrial potential probes.

    Cell death and differentiation·2005
    Same author

    Mitochondria: gateway for cytoprotection.

    Circulation research·2001
    Same author

    Restoration of Ca2+-inhibited oxidative phosphorylation in cardiac mitochondria by mitochondrial Ca2+ unloading.

    Molecular and cellular biochemistry·2001
    Same author

    Increased calcium vulnerability of senescent cardiac mitochondria: protective role for a mitochondrial potassium channel opener.

    Mechanisms of ageing and development·2001
    Same author

    Diazoxide protects mitochondria from anoxic injury: implications for myopreservation.

    The Journal of thoracic and cardiovascular surgery·2001
    Same author

    Mitochondrial K(ATP) channels: probing molecular identity and pharmacology.

    Journal of molecular and cellular cardiology·2000

    Area of Science:

    • Mitochondrial physiology
    • Biophysics of cellular structures

    Background:

    • Mitochondria are crucial for cellular energy production.
    • The dynamic behavior of mitochondrial suspensions can lead to complex spatial patterns.
    • Understanding mitochondrial function requires investigating their structural organization.

    Purpose of the Study:

    • To investigate the formation of spatial structures in unstirred mitochondrial suspensions.
    • To determine the factors influencing mitochondrial pattern development.
    • To elucidate the role of ion transport systems in spatial organization.

    Main Methods:

    • Studying mitochondrial suspensions in thin, unstirred layers.
    • Monitoring pattern development in relation to mitochondrial activity.
    • Utilizing inhibitors of respiratory chain and chelators (EGTA, EDTA) to probe ion transport mechanisms.

    Main Results:

    • Spatial structure formation is dependent on the state of mitochondrial ion-transporting systems.
    • Pattern development correlates with the activation of cation efflux from mitochondria.
    • The process is energy-dependent and sensitive to respiratory chain inhibitors.
    • Inhibition by EGTA, EDTA, and ruthenium red indicates a requirement for divalent cation translocation.

    Conclusions:

    • Mitochondrial spatial patterning is an active, energy-dependent process.
    • Divalent cation translocation is essential for the observed phenomenon.
    • The findings provide insights into the biophysical mechanisms governing mitochondrial organization.

    Related Experiment Videos