Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence specificity of curved DNA.

S Diekmann

    FEBS Letters
    |January 20, 1986
    PubMed
    Summary

    DNA fragment migration anomalies on gels indicate curvature. Repeating adenine sequences (dA)n with n≥4 cause significant curvature, supporting a B

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

    Physical review letters·2026
    Same author

    First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

    Physical review letters·2026
    Same author

    Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

    Physical review letters·2026
    Same author

    Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

    Physical review letters·2026
    Same author

    Observation of tWZ Production at the CMS Experiment.

    Physical review letters·2026
    Same author

    First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

    Physical review letters·2026

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Genetics

    Background:

    • Anomalously slow migration of DNA fragments during polyacrylamide gel electrophoresis suggests DNA curvature.
    • Existing models propose different mechanisms for DNA curvature, requiring experimental validation.

    Purpose of the Study:

    • To synthesize, clone, and electrophoretically characterize DNA fragments to differentiate between models of DNA curvature.
    • To investigate the relationship between specific DNA sequences and observed electrophoretic anomalies.

    Main Methods:

    • Synthesis and cloning of various DNA fragments.
    • Electrophoretic characterization of synthesized DNA fragments on polyacrylamide gels.
    • Analysis of migration patterns to identify anomalies indicative of DNA curvature.

    Main Results:

    • Significant migration anomalies were observed for DNA fragments containing (dA)n repeats in phase with the helical turn, where n is at least 4.
    • Fragments with (dA)n repeats where n < 4 exhibited negligible anomalies.
    • The findings contradict the purine-clash hypothesis as an explanation for DNA curvature.

    Conclusions:

    • Longer stretches of adenine (A) in a B'-form DNA structure are likely responsible for observed DNA curvature.
    • The tilt of the B'-form structure is proposed as the mechanism causing the curvature.
    • Electrophoretic analysis provides a method to distinguish between DNA curvature models.

    Related Experiment Videos