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Updated: Jun 9, 2025

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Published on: January 12, 2021
TCRosetta: An Integrated Analysis and Annotation Platform for T-cell Receptor Sequences.
Tao Yue1, Si-Yi Chen1, Wen-Kang Shen1
1Center for Artificial Intelligence Biology, Hubei Bioinformatics & Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
TCRosetta is a new online tool for analyzing T-cell receptor (TCR) repertoires. It simplifies complex TCR sequence analysis, offering comprehensive features for immune response and immunotherapy research.
Area of Science:
- Immunology
- Bioinformatics
Background:
- T cells and T-cell receptors (TCRs) are crucial for adaptive immunity.
- TCR repertoire characterization is vital for understanding immune responses and immunotherapy.
- Existing online tools for TCR repertoire analysis are limited, especially for advanced sequence annotation.
Purpose of the Study:
- To develop a comprehensive and user-friendly online server for T-cell receptor repertoire analysis.
- To integrate diverse analytical methods for simplified TCR data interpretation.
Main Methods:
- Development of TCRosetta, a web server integrating general feature analysis, sequence clustering, network construction, peptide-TCR binding prediction, generation probability calculation, and k-mer motif analysis.
- The server accepts multiple input formats and processes approximately 20,000 TCR sequences in under 3 minutes.
Main Results:
- TCRosetta provides a unified platform for various TCR repertoire analyses.
- The server offers efficient and rapid analysis of large TCR sequence datasets.
- It facilitates advanced analyses including sequence annotation and visualization.
Conclusions:
- TCRosetta is a comprehensive and efficient online tool for T-cell receptor repertoire analysis.
- It simplifies complex data analysis, supporting research in immunology and immunotherapy.
- The freely available server enhances accessibility for researchers worldwide.
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