Related Experiment Video
Updated: Jun 21, 2026

Preparation of Viral DNA from Nucleocapsids
Published on: August 16, 2011
Logistics for Rapid Isolation of Viruses From Humans.
Philip Bacchus1, Wanda Christ1, Arian Frisell1
1Philip Bacchus is a Commander, Alexander Potapeiko and Anna-Clara Markström are Privates First Class, and Felicia Leopoldson is a Corporal; all at the National CBRN Defence Centre, Swedish Armed Forces, Umeå, Sweden. Philip Bacchus is also a PhD Student, and David Gisselsson, MD, PhD, is a Senior Consultant and Professor; both in the Division of Clinical Genetics, Department of Laboratory Medicine, Lund University, Lund, Sweden. Arian Frisell is a Biomedical Scientist; Ulrika Marking, MD, MPH, is an Infectious Disease Analyst; Andreas Bråve, PhD, is Deputy Head; Kim Blom, PhD, is a Project Manager and Immunologist; and Ramona Groenheit, PhD, is a Microbiologist; all in the Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden. Ulrika Marking is also a PhD Student; Nina Greilert-Norin, RN, is a Research Nurse; Sebastian Havervall, MD, is Consultant and a PhD Student; and Charlotte Thålin, MD, PhD, is Affiliated to Research/Docent; all in the Department of Clinical Sciences, Danderyd Hospital, Karolinska Institutet, Stockholm, Sweden. Wanda Christ, MSc, PhD, is a Postdoctoral Researcher, Department of Micorbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. Jonas Klingström, PhD, is a Professor, Division of Molecular Medicine and Virology, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden.
Public health agencies need live viruses for crucial microneutralization assays. Sweden developed a rapid protocol during the COVID-19 pandemic to collect samples and isolate novel virus variants for research and diagnostics.
Area of Science:
- Microbiology
- Virology
- Public Health
Background:
- Access to live viruses is essential for microbiological surveillance.
- Microneutralization assays require live viruses for studying viral characteristics and developing diagnostic controls.
- The COVID-19 pandemic highlighted the need for rapid virus isolation protocols.
Purpose of the Study:
- To establish a protocol for the rapid collection of clinical samples.
- To enable the isolation of novel virus variants.
- To support microbiological surveillance and diagnostic development.
Main Methods:
- Development of a rapid sample collection protocol.
- Implementation of virus isolation techniques for novel variants.
- Utilizing established microbiological surveillance frameworks.
Main Results:
- A functional protocol for rapid clinical sample collection was established.
- Successful isolation of novel virus variants was achieved.
- The protocol supports the production of live virus for assays and controls.
Conclusions:
- The developed protocol facilitates efficient virus isolation for public health surveillance.
- Rapid isolation of live virus variants is critical for understanding emerging infectious diseases.
- This methodology enhances the capacity for in vitro viral research and diagnostic test development.
Related Concept Videos
Viral Recombination
Infection
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
Transmission-based Precautions I: Contact, Enteric, and Droplets
Contact Precautions:
Contact precautions are the measures taken to prevent the transmission of infectious agents, especially epidemiologically important microorganisms such as MRSA or influenza, primarily transmitted through direct or indirect contact with an...
Reservoir of Infection
Transmission of Pathogens
Coronavirus

