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Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

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The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
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  9. Predictive And Prognostic Markers
  11. Long Noncoding Vim-as1: Biomarker Of Breast Fibrosis Susceptibility After Radiation Therapy And Promoter Of Transforming Growth Factor Beta1-driven Fibrosis

Long Noncoding VIM-AS1: Biomarker of Breast Fibrosis Susceptibility After Radiation Therapy and Promoter of Transforming Growth Factor Beta1-Driven Fibrosis

Tatiana Vinasco-Sandoval1, Sandra Moratille1, Françoise Crechet1

  • 1CEA, Laboratoire de Génomique et Radiobiologie de la Kératinopoïèse (LGRK), Evry, France; Université Paris-Saclay, France; CEA, Institut de Biologie François Jacob (IBFJ), Département de Radiobiologie Cellulaire et Moléculaire (DRCM), Fontenay-aux-Roses, France.

International Journal of Radiation Oncology, Biology, Physics
|October 22, 2024

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Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
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View abstract on PubMed

Summary
This summary is machine-generated.

This study identifies noncoding RNAs, specifically VIM-AS1, as key biomarkers for predicting radiation-induced fibrosis. Targeting these long noncoding RNAs offers new therapeutic avenues for managing fibrotic complications.

Area of Science:

  • Genomics
  • Molecular Biology
  • Oncology

Background:

  • Radiation therapy for cancer can lead to late complications like fibrosis.
  • While coding genes are studied, the role of noncoding RNA in fibrosis is less understood.
  • The TGFB1 gene network is implicated in fibrosis development.

Purpose of the Study:

  • To investigate the role of the noncoding genome in radiation-induced fibrosis susceptibility.
  • To identify noncoding RNA biomarkers for predicting fibrosis development after radiation therapy.

Main Methods:

  • Retrospective analysis of breast cancer patients with and without severe fibrosis.
  • Exome sequencing and RNA-seq on dermal fibroblasts.
  • Functional experiments including gene knockdown in healthy fibroblasts.

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Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
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Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
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Main Results:

  • A 15-long noncoding RNA (lncRNA) signature for breast fibrosis susceptibility was identified.
  • Vimentin antisense long noncoding RNA 1 (VIM-AS1) emerged as a significant fibrosis risk biomarker.
  • VIM-AS1 demonstrated profibrotic activity, influencing fibroblast-myofibroblast transition via the TGFB1/VIM-AS1/vimentin axis.

Conclusions:

  • Noncoding RNA analysis provides predictive biomarkers for radiation therapy responses.
  • This opens avenues for next-generation, RNA-based therapeutic strategies.
  • VIM-AS1 may play a general role in various fibrotic disorders.