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Related Concept Videos

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  3. Screening And Application Of Aptamers As Fluorescent Biosensors For Selective And Sensitive Detection Of Hepatocellular Carcinoma And In Vivo Targeted Delivery Studies.
  1. Home
  3. Screening And Application Of Aptamers As Fluorescent Biosensors For Selective And Sensitive Detection Of Hepatocellular Carcinoma And In Vivo Targeted Delivery Studies.

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Screening and application of aptamers as fluorescent biosensors for selective and sensitive detection of

Xuyan Liu1, Lei He2, Zhenxing Fan3

  • 1Hebei Center for Ecological and Environmental Geology Research, Hebei GEO University, Shijiazhuang, 050031, China. xclxy0203@163.com.

Mikrochimica Acta
|October 22, 2024

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
Circular dichronismEarly HCC diagnosisFlow cytometryFluorescent biosensorsLuciferase mRNANucleic acid aptamers

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New aptamers show high affinity and specificity for hepatocellular carcinoma (HCC) detection. These aptamers enable early diagnosis and targeted liver delivery, demonstrating significant potential for clinical applications in HCC treatment.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Oncology

Background:

  • Hepatocellular carcinoma (HCC) incidence is rising globally, making early diagnosis critical.
  • Current diagnostic methods require improvement for timely intervention.

Purpose of the Study:

  • To develop novel aptamers for specific and sensitive detection of HCC.
  • To evaluate the potential of these aptamers for early diagnosis and targeted therapy.

Main Methods:

  • Magnetic bead SELEX technology was used to identify anti-HCC aptamers.
  • Flow cytometry, circular dichroism, and molecular docking were employed for characterization.
  • Aptamer sensing performance and in vivo delivery were analyzed.

Main Results:

  • Apt-1 exhibited the highest affinity (Kd = 5.9 nM) and specific recognition of HCC serum.
  • Aptamers formed stem-loop B-DNA structures, with binding dominated by hydrogen bonds.
  • The developed aptamer assay achieved a limit of detection of 0.75 nM and >90% positive detection in clinical samples.

Conclusions:

  • The novel aptamers demonstrate high specificity and sensitivity for HCC detection.
  • Aptamer-mediated delivery successfully targeted the liver in vivo.
  • These findings support the clinical application of aptamers for early HCC diagnosis and potential therapeutic strategies.