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Establishment of an induced pluripotent cell line (ABCRIi001-A) from an elderly female for ageing research

Filipa Esteves1, David Brito1, Ana Teresa Rajado1

  • 1Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal.

Stem Cell Research
|October 22, 2024
PubMed

Insights

We generated a new human induced pluripotent stem cell (hiPSC) line, ABCRIi001-A, from an elderly female donor. This valuable cell line can be used for studying age-related diseases and developing aging scores.

Area of Science:

  • Stem cell biology
  • Gerontology
  • Disease modeling

Background:

  • Human induced pluripotent stem cells (hiPSCs) are crucial for disease modeling, particularly for age-related conditions.
  • Understanding the aging process requires robust cellular models derived from elderly individuals.

Purpose of the Study:

  • To generate and characterize a novel hiPSC line from a 79-year-old female donor.
  • To establish a valuable cellular resource for aging research and the development of aging scores.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) from an elderly female donor were reprogrammed using Sendai virus vectors (hKOS, hc-Myc, hKlf4).
  • The generated hiPSC line (ABCRIi001-A) underwent rigorous characterization, including morphology, karyotype, viral clearance, and pluripotency assays.

Main Results:

  • The ABCRIi001-A hiPSC line exhibited normal morphology and karyotype.
  • Confirmed viral clearance, absence of genomic aberrations, and successful differentiation into three germ layers.
  • Pluripotency was validated through the expression of key markers.

Conclusions:

  • The ABCRIi001-A hiPSC line is a well-characterized and stable cellular model.
  • This hiPSC line is a valuable resource for investigating human aging mechanisms and diseases.

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