CRISPR-Cas systems in enterococci
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Enterococci harbor CRISPR-Cas systems, a defense mechanism against antimicrobial resistance genes. These systems, particularly CRISPR2, show potential as gene editing tools in enterococci research.
Area Of Science
- Microbiology
- Genetics
- Molecular Biology
Background
- Enterococci are common microbiota in humans and animals, prevalent in environments and healthcare settings.
- Widespread antimicrobial use drives resistance in enterococci, posing a significant public health challenge within the One Health framework.
- CRISPR-Cas systems offer prokaryotic defense against mobile genetic elements, potentially limiting antimicrobial resistance and virulence gene acquisition.
Purpose Of The Study
- To review CRISPR-Cas systems in enterococci, examining their prevalence, structure, function, and applications.
- To understand the role of CRISPR-Cas in enterococcal antimicrobial resistance and virulence.
- To explore the utility of CRISPR-Cas as a gene-editing technology in enterococci.
Main Methods
- Literature review of studies on CRISPR-Cas systems in enterococci.
- Analysis of CRISPR system occurrence, structure (spacers, direct repeats), and organization.
- Examination of CRISPR-Cas mechanisms and applications in enterococcal gene editing.
Main Results
- Type II-A CRISPR-Cas systems are most frequent in enterococci.
- The orphan CRISPR2 system is the most common (54.1%), particularly in Enterococcus faecalis.
- CRISPR systems exhibit variation in distribution, spacer count (1-20), spacer size (23-37 bp), and direct repeat length (25-37 bp).
Conclusions
- CRISPR-Cas systems are present in enterococci with diverse characteristics.
- CRISPR-Cas technology offers a promising tool for targeting resistance and virulence genes in enterococci.
- Further research into CRISPR-Cas in enterococci can advance antimicrobial resistance and gene editing strategies.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...