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Published on: August 15, 2011
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A spatiotemporal transcriptomic atlas of mouse placentation
Yanting Wu1,2,3,4, Kaizhen Su5,6,7, Ying Zhang8,9
1Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China. yanting_wu@163.com.
Cell Discovery
|October 22, 2024
Summary
This study maps gene expression in the mouse placenta across gestation, revealing key genes and pathways involved in development and function. Findings highlight how maternal diet impacts placental health, offering insights into pregnancy complications.
Area of Science:
- Developmental Biology
- Genomics
- Reproductive Biology
Background:
- The placenta is crucial for pregnancy, but its gene expression patterns during development are not fully understood.
- Previous studies lacked detailed spatiotemporal resolution and coverage of all cell types.
Purpose of the Study:
- To create a high-resolution spatiotemporal transcriptomic atlas of mouse placentation.
- To identify key genes and pathways regulating placental development, angiogenesis, and function.
- To investigate the impact of maternal diet on placental health.
Main Methods:
- Utilized Stereo-seq technology for high-resolution spatial transcriptomics.
- Analyzed gene expression from embryonic day 7.5 to 14.5.
- Performed spatiotemporal ligand-receptor interaction analysis and transcription factor regulon identification.
Main Results:
- Developed the Mouse Placentation Spatiotemporal Transcriptomic Atlas (MPSTA).
- Defined early trophoblast strata and mapped differentiation trajectories.
- Identified essential modulators of trophoblast development and placental angiogenesis.
- Discovered interface-specific transcription factors crucial for gestational maintenance.
- Found that maternal high-fat diet disrupts angiogenic homeostasis and increases inflammation.
Conclusions:
- The MPSTA provides a comprehensive spatial map of placental gene expression.
- Pivotal regulators of placental development and function have been identified.
- Maternal diet significantly impacts placental interface integrity and homeostasis.
