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Updated: Jun 9, 2025
Author Spotlight: Establishing an Accurate Microhardness Testing Protocol for Craniofacial Tissues
Published on: April 26, 2024
Re-evaluation of Microhardness of Mineral Trioxide Aggregate and Biodentine After Removal From Root Using Different
Ruchali S Bhandare1, Sudha B Mattigatti1
1Department of Conservative Dentistry and Endodontics, School of Dental Science, Krishna Vishwa Vidyapeeth, Karad, IND.
Abstract:
Objective This study aimed to evaluate the effects of different solvents on the microhardness of mineral trioxide aggregate (MTA) and Biodentine, which are used in endodontic therapy for their biocompatibility and ability to create a hermetic seal. Methods A total of 150 extracted human single-rooted teeth were selected and sectioned into 4 mm slices. The samples were divided into two groups according to the material used: MTA and Biodentine. Then the samples were incubated at 37°C and 100% humidity for 28 days. After that, 15 MTA and Biodentine samples each were sent for microhardness test and the remaining samples were subdivided into four subgroups according to the solvent used, i.e., treated with normal saline, 5.25% sodium hypochlorite, 10% citric acid, or 40% hydrofluoric acid. The microhardness of the upper and lower surfaces of the samples was measured using a Vickers microhardness tester. Results The average microhardness values for Biodentine and MTA were found to be 74.4 HV and 69.9 HV, respectively. Treatment with sodium hypochlorite, citric acid, and hydrofluoric acid significantly reduced the microhardness of both materials. Hydrofluoric acid had the most pronounced effect, followed by citric acid and sodium hypochlorite. Statistical analysis using one-way analysis of variance (ANOVA) 'F' test showed highly significant differences between the groups. Discussion The study demonstrated that solvents such as hydrofluoric acid, citric acid, and sodium hypochlorite effectively reduced the microhardness of MTA and Biodentine, facilitating their removal during retreatment. This reduction in microhardness is attributed to the chemical interactions of the solvents with the materials, leading to disintegration and reduced structural integrity. Conclusion Biodentine had greater microhardness compared to MTA, and hydroflouric acid reduced the microhardness to a greater extent compared to other solvents.
