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Related Experiment Video

Updated: Jun 9, 2025

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
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Endosomal membrane budding patterns in plants.

Ethan Weiner1, Elizabeth Berryman1, Felix Frey2

  • 1Center for Quantitative Cell Imaging and Department of Botany, University of Wisconsin-Madison, Madison, WI 53706.

Proceedings of the National Academy of Sciences of the United States of America
|October 23, 2024
PubMed
Summary
This summary is machine-generated.

Plant endosomes form complex intralumenal vesicle (ILV) networks, driven by Endosomal Sorting Complex Required for Transport (ESCRT) proteins. This study reveals the evolution and molecular drivers of these concatenated ILV networks in plants.

Keywords:
ESCRTmultivesicular endosomesvesiculation

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Area of Science:

  • Cell Biology
  • Plant Biology
  • Molecular Biology

Background:

  • Multivesicular endosomes (MVEs) use Endosomal Sorting Complex Required for Transport (ESCRT) proteins to sequester proteins into intralumenal vesicles (ILVs) for degradation.
  • In Arabidopsis, uncoupled membrane constriction and scission lead to concatenated ILV networks, enhancing cargo sequestration.

Purpose of the Study:

  • To investigate the evolutionary timeline of concatenated ILV networks in plants.
  • To identify the molecular mechanisms driving the formation of these networks.

Main Methods:

  • Electron tomography and morphometric analysis of endosomes from diverse plant species and yeast.
  • Particle-based molecular dynamics simulations of ESCRT protein interactions.
  • Computational modeling of membrane remodeling and ILV network topology.

Main Results:

  • Concatenated ILV networks are widespread in plants, particularly prevalent in seed and flowering plants.
  • Independent evolution of multiple budding sites in hornworts and seed plants.
  • ESCRT filament properties (curvature, binding energy) and membrane remodeling parameters influence network formation.

Conclusions:

  • The evolution of concatenated ILV networks is linked to specific plant lineages and ESCRT-mediated membrane remodeling.
  • Multiple budding sites represent an independently evolved mechanism for ILV formation in plants.
  • Computational models successfully recapitulate observed ILV network structures and formation dynamics.