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Synthesis and Application of D- and 13C-Labelled tert-Butyl Hoechst Dye
Yulia A Vlasenko1, Avery J To1, Tess Fortier1
1Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada.
Abstract:
Herein, the successful syntheses of D3- and 13C-N-methyl and D9-tert-butyl Hoechst dyes are presented. This includes the preparation of the labelled D3- and 13C-N-methyl piperazines and D9-tert-butylated hydroxytoluene precursors. The tert-butyl Hoechst dye is known to bind a specific RNA aptamer. Spectroscopic NMR studies of the labelled Hoechst dye-aptamer complexes allowed for the unambiguous assignment of chemical shifts, as well as the dynamics of the bound dye.

