Related Experiment Video
Updated: Jun 9, 2025
07:46
Assessing Endothelial Vasodilator Function with the Endo-PAT 2000
Published on: October 15, 2010
43.7K
Effect of 5β-dihydrotestosterone on vasodilator function and on cell proliferation
David Sánchez-Fernández1, Aritz Eguibar2, Cristina López1
1Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain.
Plos One
|October 23, 2024
Summary
5β-dihydrotestosterone (5β-DHT) enhances blood vessel function and antioxidant capacity in aged rats without increasing prostate tumor cell growth. This makes 5β-DHT a potential therapeutic for cardiovascular diseases.
Area of Science:
- Gerontology
- Cardiovascular Medicine
- Endocrinology
Background:
- Aging is a primary risk factor for cardiovascular diseases.
- Androgens, like testosterone (TES), offer cardiovascular benefits but are contraindicated in prostate cancer.
- 5β-dihydrotestosterone (5β-DHT), a TES metabolite, lacks genomic effects and may offer therapeutic potential.
Purpose of the Study:
- To investigate the effects of 5β-DHT on vascular function and cell proliferation.
- To assess 5β-DHT's impact on vasodilator function and antioxidant actions in aged rats.
- To determine 5β-DHT's influence on prostate tumor and vascular smooth muscle cell proliferation.
Main Methods:
- Utilized mesenteric arteries from aged-orchidectomized Sprague-Dawley rats.
- Administered androgens (10 nM) to arterial segments to analyze responses to acetylcholine and sodium nitroprusside.
- Assessed nitric oxide and superoxide anion production, and androgen effects on cell viability.
Main Results:
- 5β-DHT significantly improved vasodilator function in aged rat arteries.
- 5β-DHT demonstrated antioxidant effects.
- Prostate tumor cell proliferation rates remained unaffected by 5β-DHT treatment.
Conclusions:
- 5β-DHT enhances vascular function and exhibits antioxidant properties.
- 5β-DHT does not stimulate the proliferation of androgen-dependent prostate tumor cells.
- 5β-DHT presents a promising therapeutic candidate for cardiovascular pathologies.
More Related Videos
08:41Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues
Published on: June 3, 2019
9.8K
09:33A Methodological Approach to Non-invasive Assessments of Vascular Function and Morphology
Published on: February 7, 2015
16.3K
Related Concept Videos
01:23Antihypertensive Drugs: Vasodilators
483
Vasodilators, primarily affecting the smooth muscles within arterial and venous walls, are commonly used for hypertension treatment. Medications such as minoxidil and hydralazine primarily target arteries and arterioles, while sodium nitroprusside acts on arterioles and venules. Minoxidil, functioning as a prodrug, is metabolized by hepatic sulfotransferase into its active form, minoxidil sulfate, after oral administration. This metabolite binds to the sulfonylurea receptor (SUR) component of...
483
01:28Nitric Oxide Signaling Pathway
4.9K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
4.9K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
01:28Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors
129
Phosphodiesterase 5 (PDE5) inhibitors are potent enzymes that function to hydrolyze cyclic nucleotides to their corresponding 5' monophosphates. Their unique biochemical properties have been applied in treating Pulmonary Arterial Hypertension (PAH).
Among the PDE5 inhibitors, sildenafil (Revatio) stands out as a competitive and selective inhibitor. It operates by elevating cellular levels of cGMP and augmenting signaling through the cGMP-PKG pathway, promoting vasodilation. Upon oral...
Among the PDE5 inhibitors, sildenafil (Revatio) stands out as a competitive and selective inhibitor. It operates by elevating cellular levels of cGMP and augmenting signaling through the cGMP-PKG pathway, promoting vasodilation. Upon oral...
129
01:22Target Cell Response to Hormones
2.9K
Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
2.9K
01:26Testosterone: Functions and Regulation
566
The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...
566