Integrating rhizosphere bacterial structure and metabolites with soil Cd availability in different parent paddy soils

Soil bacterial community structure and rhizosphere metabolites are important pathways for rice to respond to external Cd stress. The specific correlations between these microorganisms, metabolites and inherent soil properties, as well as the mechanisms they utilize to regulate Cd availability across different parent soils remain underexplored, emphasizing the need for deeper understanding to inform effective soil management strategies. In this study, five typical parent soils with large differences in properties (quaternary red clay soil (hereby defined as Q), granite soil (G), river sandy mud (R), yellow mud soil (Y), stucco field (S)) in Chinese paddy soils were collected, and extra Cd were added (CK: 0 mg·kg^-1, Cd: 2.4 mg·kg^-1).The result indicated that the toxicity impact of Cd in rice grains in G was the weakest, and the highest Cd bioavailability in S. The abundance of Proteobacteria, Bacteroidota and Firmicutes showed an increasing trend in G, while they decreased significantly in S. The contents of Cis-9-palmitoleic acid and phosphoethanolamine increased by 170.02 % and 154.03 % in G, decreased by 218.62 % and 181.58 % in S. MBNT15 and Desulfobacterota showed a significant negative correlation with humic acid molecular weight (MW) extracted from parent soils and Clay (montmorillonite, illite, kaolinite) contents, while they exhibited a positive correlation with soil organic matter (OM) content (P < 0.01). The MW played a crucial role in shaping rhizosphere metabolites with R² value of 0.8498. These results elucidate how soil bacterial communities, rhizosphere metabolites, and inherent soil properties interact to regulate Cd availability across different parent soils.