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Screening and analysis of GULP1 downstream target genes based on transcriptomic sequencing.

Xin Wen1, Jin Mei1, Mei-Yu Qian1

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Yi Chuan = Hereditas
|October 23, 2024
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Summary

GULP1 protein influences glucose uptake and impacts lipid and cholesterol metabolism. This study identified key genes and pathways affected by GULP1 overexpression in adipocytes and skeletal muscle cells.

Keywords:
GULP1bioinformaticdifferentially expressed genemetabolismtranscriptome

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Area of Science:

  • Molecular Biology
  • Metabolic Research
  • Cellular Biology

Background:

  • GULP1 (engulfment adaptor protein) with a PTB domain promotes glucose uptake in adipocytes.
  • Understanding GULP1's downstream metabolic effects is crucial for metabolic research.

Purpose of the Study:

  • To identify key metabolically related differential genes downstream of GULP1.
  • To analyze transcriptome data from adipocytes and skeletal muscle cells overexpressing GULP1.

Main Methods:

  • Transcriptome analysis of adipocytes and skeletal muscle cells overexpressing GULP1.
  • Bioinformatic analysis of differentially expressed genes.
  • Validation using real-time fluorescent quantitative PCR (qRT-PCR).

Main Results:

  • Overexpression of GULP1 in adipocytes led to 278 upregulated and 263 downregulated genes, impacting cholesterol and lipid metabolism.
  • In skeletal muscle cells, GULP1 overexpression resulted in 280 upregulated and 302 downregulated genes, affecting amino acid, lipid, and carbohydrate metabolism.
  • Enrichment analysis identified key KEGG pathways related to lipid, amino acid, and carbohydrate metabolism.

Conclusions:

  • GULP1 plays a significant role in regulating cellular metabolism, particularly lipid metabolism.
  • The study provides a foundation for further research into GULP1's downstream targets and functions in metabolic processes and oncology.