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Related Experiment Video

Updated: Jun 9, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

Real-time and programmable transcriptome sequencing with PROFIT-seq.

Jinyang Zhang¹, Lingling Hou¹, Lianjun Ma²

¹Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Nature Cell Biology

|October 24, 2024

Summary

This summary is machine-generated.

Introducing PROFIT-seq, a novel method for transcriptome sequencing that enriches target RNA while maintaining whole transcriptome quantification. This advance improves data yield and speeds up detection of specific pathogens or mutations.

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Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

Eukaryotic transcriptome complexity hinders specific transcript detection.
Existing targeted RNA sequencing methods require complex enrichment, potentially compromising comprehensive analysis.

Purpose of the Study:

To introduce programmable full-length isoform transcriptome sequencing (PROFIT-seq) for targeted transcript enrichment and whole transcriptome quantification.
To overcome limitations of current methods in RNA sequencing.

Main Methods:

PROFIT-seq utilizes combinatorial reverse transcription to capture various RNA types (polyadenylated, non-polyadenylated, circular).
A programmable control system selectively enriches target transcripts during sequencing.
The method ensures unbiased quantification of the entire transcriptome.

Main Results:

PROFIT-seq increases effective data yield by over 3-fold.
It reduces the time for detecting specific pathogens or mutations by 75%.
Application in colorectal polyp development revealed host immune response and bacterial infection interplay.

Conclusions:

PROFIT-seq provides accurate and efficient sequencing of target transcripts while preserving global transcriptome quantification.
The method has broad applications in clinical diagnostics and targeted enrichment.