Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

9.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Survey-based insights on overcoming barriers in healthcare innovation ecosystems.

Nature biotechnology·2026
Same author

Aging disrupts spatiotemporal coordination in the cycling murine ovary.

Nature aging·2026
Same author

Long-term isolation and archaic introgression shape functional genetic variation in Near Oceania.

Science (New York, N.Y.)·2026
Same author

Rapid development and field evaluation of a portable CRISPR-based assay for Mpox during the 2025 Sierra Leone outbreak.

Nature communications·2026
Same author

"Painless palsy" revisited: a systematic review of pain in hereditary neuropathy with liability to pressure palsies.

Pain management·2026
Same author

Rapid Cas13a-based <i>pen</i>A genotyping for cefixime susceptibility in <i>Neisseria gonorrhoeae</i>.

mSphere·2026

Related Experiment Video

Updated: Jun 9, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.4K

Machine-guided design of cell-type-targeting cis-regulatory elements.

Sager J Gosai1,2,3,4, Rodrigo I Castro5, Natalia Fuentes6,7

  • 1Broad Institute of MIT and Harvard, Cambridge, MA, USA. sgosai@broadinstitute.org.

Nature
|October 24, 2024
PubMed
Summary

Scientists engineered synthetic cis-regulatory elements (CREs) to precisely control gene expression. These novel CREs demonstrate superior cell-type specificity compared to natural sequences, offering potential for targeted gene therapies and biotechnology.

More Related Videos

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.0K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.3K

Related Experiment Videos

Last Updated: Jun 9, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.4K
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.0K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.3K

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioengineering

Background:

  • Cis-regulatory elements (CREs) are crucial for controlling gene expression and defining cell types.
  • Natural CREs may not be optimal for therapeutic applications requiring precise tissue specificity.

Purpose of the Study:

  • To develop a platform for engineering and validating synthetic CREs with programmed cell-type specificity.
  • To create novel regulatory elements for potential use in gene therapy and biotechnology.

Main Methods:

  • Utilized deep neural network modeling to predict CRE activity.
  • Employed in silico optimization and massively parallel reporter assays (MPRAs) to design and test thousands of synthetic CREs.
  • Validated synthetic CREs in vitro using cell lines and in vivo in analogous tissues.

Main Results:

  • Synthetic CREs demonstrated enhanced cell-type-specific gene expression compared to natural human CREs in vitro.
  • In vivo testing confirmed specific expression in target tissues.
  • Engineered CREs showed distinct sequence motifs associated with on-target activity and reduced off-target activity.

Conclusions:

  • A generalizable framework was established for engineering synthetic CREs using MPRAs.
  • The study provides a method to "write" regulatory code for fit-for-purpose applications.
  • Synthetic CREs offer a promising avenue for precise control of gene expression in therapeutic and biotechnological contexts.