Related Experiment Video

Updated: Jun 9, 2025

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
07:58

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Published on: May 30, 2017

10.9K

Molecular characterisation of virulence genes in bacterial pathogens from daycare centres in Ile-Ife, Nigeria:

Eunice Damilola Wilkie1, Jude Oluwapelumi Alao2, Toyosi Teniola Sotala3

  • 1Microbiology Department, Adeleke University, Ede, Osun State, Nigeria. wilkie.eunice@adelekeuniversity.edu.ng.

BMC Infectious Diseases
|October 24, 2024
PubMed

Insights

Virulent bacteria, including Staphylococcus aureus and Klebsiella, were found in Nigerian daycare centers, posing infection risks to children. Enhanced hygiene and screening are crucial to protect young children's health.

Area of Science:

  • Microbiology and Public Health

Background:

  • Daycare centers are crucial for child development but pose high risks for infectious disease transmission.
  • Limited resources in low- and middle-income countries (LMICs) like Nigeria exacerbate infection control challenges.

Purpose of the Study:

  • To identify and characterize virulence genes in bacterial isolates from Nigerian daycare centers.
  • To assess the potential infection risks posed by these bacteria to young children.

Main Methods:

  • Collected 233 samples from children, staff, and fomites across 17 daycare centers.
  • Utilized conventional PCR and RAPD analysis to detect virulence genes in bacterial isolates.
  • Recorded the frequency of key virulence genes and the prevalence of bacterial species.

Main Results:

  • Prevalent bacterial species included Staphylococcus aureus (35%), Klebsiella (28%), and Pseudomonas aeruginosa (20%).
  • Detected key virulence genes: fimH in Klebsiella (22.73%), algD in P. aeruginosa (50%), and icaA/cna in S. aureus (16.67%).

Conclusions:

  • Virulent bacterial pathogens are present in daycare environments, posing significant infection risks to children.
  • Enhanced infection control measures, including microbial screening, hygiene, and disinfection, are essential.
  • Training for daycare workers and routine monitoring are vital to reduce transmission and protect child health.
Abstract

Keywords:
Klebsiella pneumoniaeStaphylococcus aureusDaycare centresNigeriaVirulence genes

More Related Videos

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

8.8K
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

6.3K

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
3
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
Infection01:20

Infection

When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
7.7K
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...