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Updated: Jun 9, 2025
Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
Gene Augmentation Techniques to Stimulate Wound Healing Process: Progress and Prospects
Jyotsana Dwivedi1, Shubhi Kaushal1, D Jeslin2
1PSIT- Pranveer Singh Institute of Technology (Pharmacy), Kanpur, India.
Abstract:
Gene therapy has traditionally been used to treat individuals with late-stage cancers or congenital abnormalities. Numerous prospects for therapeutic genetic modifications have emerged with the discovery that gene therapy applications are far more extensive, particularly in skin and exterior wounds. Cutaneous wound healing is a complex, multistep process involving multiple steps and mediators that operate in a network of activation and inhibition processes. This setting presents a unique obstacle for gene delivery. Many gene delivery strategies have been developed, including liposomal administration, high-pressure injection, viral transfection, and the application of bare DNA. Among several gene transfer techniques, categorical polymers, nanoparticles, and liposomalbased constructs show great promise for non-viral gene transfer in wounds. Clinical experiments have shown that efficient transportation of certain polypeptides to the intended wound location is a crucial factor in wound healing. Genetically engineered cells can be used to produce and control the delivery of specific growth factors, thereby addressing the drawbacks of mechanically administered recombinant growth factors. We have discussed how repair mechanisms are based on molecules and cells, as well as their breakdown, and provided an overview of the methods and research conducted on gene transmission in tissue regeneration.
Insights
Gene therapy offers new potential for wound healing by delivering therapeutic genes to skin and exterior wounds. Advanced non-viral methods show promise for effective gene transfer and tissue regeneration.
Area of Science:
- Regenerative Medicine
- Molecular Biology
- Biotechnology
Background:
- Gene therapy traditionally targets late-stage cancers and congenital abnormalities.
- Recent discoveries highlight extensive applications in skin and wound healing.
- Cutaneous wound healing is a complex biological process involving intricate molecular signaling.
Purpose of the Study:
- To explore the potential of gene therapy in enhancing cutaneous wound healing.
- To review current gene delivery strategies for wound applications.
- To discuss the role of genetically engineered cells in controlled growth factor delivery for tissue regeneration.
Main Methods:
- Overview of various gene delivery strategies, including liposomal administration, viral transfection, and non-viral methods.
- Focus on categorical polymers, nanoparticles, and liposomal constructs for non-viral gene transfer.
- Discussion of genetically engineered cells for localized and controlled delivery of therapeutic factors.
Main Results:
- Efficient polypeptide delivery to wound sites is critical for successful healing.
- Non-viral gene transfer methods, particularly nanoparticles and liposomes, show significant promise.
- Genetically engineered cells offer a controlled approach to growth factor delivery, overcoming limitations of recombinant factors.
Conclusions:
- Gene therapy presents a promising frontier for advancing wound healing and tissue regeneration.
- Optimized gene delivery systems are essential for therapeutic success in complex wound environments.
- Further research into molecular and cellular repair mechanisms can drive innovation in regenerative medicine.
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