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Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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A CH4-Driven Ion Cloud-Stretched Approach Enables ICP-qMS for Multiplex Single-Cell Analysis.

Zhengxian Lv1, Xinli Yan1, Zhen Liu1

  • 1Department of Chemistry & the MOE Key Lab of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|October 24, 2024
PubMed
Summary

Inductively coupled plasma quadrupole mass spectrometry (ICP-qMS) can now analyze single cells. Methane gas extends ion cloud duration, enabling multiplexed detection of protein markers for cell classification and heterogeneity studies.

Keywords:
Breast Cancer CellsCH4Collision-Reaction-CellICP-qMSSingle-Cell Multiplex Analysis

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Mass Spectrometry

Background:

  • Inductively coupled plasma quadrupole mass spectrometry (ICP-qMS) is a powerful tool for elemental and biomolecular quantification in solution.
  • Analyzing intact single cells with ICP-qMS is challenging due to transient ion signals and instrument dwell times.
  • Existing ICP-qMS methods struggle with acquiring multi-m/z signals from single cells dispersed in suspension.

Purpose of the Study:

  • To overcome the limitations of ICP-qMS in single-cell analysis.
  • To enable multiplexed quantification of protein markers on individual cells.
  • To facilitate cell classification and reveal cellular heterogeneity using ICP-qMS.

Main Methods:

  • Utilized methane (CH4) gas within a collision-reaction cell (CRC) to extend the duration of single-cell ion clouds.
  • Implemented ICP-(CH4-CRC)-qMS for multi-m/z signal acquisition from single cells.
  • Applied the method for quantifying multiple phenotype protein markers on single cells.

Main Results:

  • Methane gas extended single-cell ion cloud duration to over 7,000 μs.
  • Achieved successful multi-m/z signal acquisition from single cells using ICP-qMS.
  • Enabled quantification of multiple protein markers, revealing single-cell heterogeneity and enabling subtype classification.

Conclusions:

  • The CH4-driven ion cloud stretching approach overcomes a major bottleneck in ICP-qMS single-cell analysis.
  • This method allows for unprecedented multiplexed analysis of single cells using ICP-qMS.
  • Paves the way for broader applications of ICP-qMS in single-cell research and diagnostics.