Distinct roles of two homologous kinesins in mammalian motile cilia
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Kinesin motor Kif6 is active, while Kif9 is a stationary regulator in mammalian cilia. Both kinesin-9 proteins are crucial for ciliary motility, according to new research.
Area Of Science
- Cell Biology
- Molecular Motors
- Cilia Biology
Background
- Cilia are crucial for cellular functions.
- Kinesin motor proteins are essential for intracellular transport.
- The specific roles of kinesin-9 family members in cilia remain unclear.
Purpose Of The Study
- To elucidate the distinct functions of Kif6 and Kif9 within mammalian cilia.
- To determine the contribution of Kif6 and Kif9 to ciliary motility.
Main Methods
- Utilized advanced microscopy techniques.
- Performed genetic manipulation of kinesin-9 members.
- Analyzed ciliary beat frequency and structure.
Main Results
- Kif6 was identified as an active motor protein driving ciliary function.
- Kif9 was characterized as a stationary regulator essential for ciliary stability.
- Both Kif6 and Kif9 were found to be indispensable for normal cilia motility.
Conclusions
- Kinesin-9 members Kif6 and Kif9 play complementary, essential roles in mammalian cilia.
- Kif6 acts as a motor, and Kif9 as a regulator, to ensure proper ciliary motility.
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
Related Concept Videos
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...